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rat origin l6 myoblasts  (ATCC)


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    ATCC rat origin l6 myoblasts
    PremiR transfections (25 nM) of rat <t>L6</t> myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).
    Rat Origin L6 Myoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+origin+l6+myoblasts/L6%3B+Myoblast%3B+Rat/pmc03323545-79-8-6
    Average 95 stars, based on 398 article reviews
    rat origin l6 myoblasts - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4"

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034596

    PremiR transfections (25 nM) of rat L6 myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).
    Figure Legend Snippet: PremiR transfections (25 nM) of rat L6 myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).

    Techniques Used: Transfection, Western Blot, SDS Page

    Small inhibitory RNA (25 nM) was used to deplete SMAD4 in L6 cell culture system prior to glucose transport assay using 14 C-2-deoxyglucose as described in . Both skeletal muscle cells (I & II) and cardiomyocytes cells (III) were used for this experiment. The transport assay condition is maintained in the same way as was done for . (IV) The Western Blot analysis in L6 cell-lines to show the specificity of siRNA against SMAD4. Dharmacon-designed SMARTpool siRNAs (4 sets) specific to SMAD4 and non-targeting negative control siRNA were used for this experiment according to the manufacturer's instruction.
    Figure Legend Snippet: Small inhibitory RNA (25 nM) was used to deplete SMAD4 in L6 cell culture system prior to glucose transport assay using 14 C-2-deoxyglucose as described in . Both skeletal muscle cells (I & II) and cardiomyocytes cells (III) were used for this experiment. The transport assay condition is maintained in the same way as was done for . (IV) The Western Blot analysis in L6 cell-lines to show the specificity of siRNA against SMAD4. Dharmacon-designed SMARTpool siRNAs (4 sets) specific to SMAD4 and non-targeting negative control siRNA were used for this experiment according to the manufacturer's instruction.

    Techniques Used: Cell Culture, Transport Assay, Western Blot, Negative Control

    Related Articles

    Transfection:

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4
    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.. L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.

    Western Blot:

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4
    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.. L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.

    SDS Page:

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4
    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.. L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.

    Cell Culture:

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4
    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.. L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.

    Transport Assay:

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4
    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.. L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.

    Negative Control:

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4
    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.. L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.L6 myoblast cells were maintained according to the ATCC protocol using DMEM with 4 mM L-glutamine adjusted to 0.15% bicarbonate and 0.45% glucose in addition to 10% FBS.



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    rat origin l6 myoblasts  (ATCC)
    95
    ATCC rat origin l6 myoblasts
    PremiR transfections (25 nM) of rat <t>L6</t> myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).
    Rat Origin L6 Myoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+origin+l6+myoblasts/L6%3B+Myoblast%3B+Rat/pmc03323545-79-8-6
    Average 95 stars, based on 1 article reviews
    rat origin l6 myoblasts - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    PremiR transfections (25 nM) of rat L6 myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).

    Journal: PLoS ONE

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4

    doi: 10.1371/journal.pone.0034596

    Figure Lengend Snippet: PremiR transfections (25 nM) of rat L6 myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).

    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.

    Techniques: Transfection, Western Blot, SDS Page

    Small inhibitory RNA (25 nM) was used to deplete SMAD4 in L6 cell culture system prior to glucose transport assay using 14 C-2-deoxyglucose as described in . Both skeletal muscle cells (I & II) and cardiomyocytes cells (III) were used for this experiment. The transport assay condition is maintained in the same way as was done for . (IV) The Western Blot analysis in L6 cell-lines to show the specificity of siRNA against SMAD4. Dharmacon-designed SMARTpool siRNAs (4 sets) specific to SMAD4 and non-targeting negative control siRNA were used for this experiment according to the manufacturer's instruction.

    Journal: PLoS ONE

    Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4

    doi: 10.1371/journal.pone.0034596

    Figure Lengend Snippet: Small inhibitory RNA (25 nM) was used to deplete SMAD4 in L6 cell culture system prior to glucose transport assay using 14 C-2-deoxyglucose as described in . Both skeletal muscle cells (I & II) and cardiomyocytes cells (III) were used for this experiment. The transport assay condition is maintained in the same way as was done for . (IV) The Western Blot analysis in L6 cell-lines to show the specificity of siRNA against SMAD4. Dharmacon-designed SMARTpool siRNAs (4 sets) specific to SMAD4 and non-targeting negative control siRNA were used for this experiment according to the manufacturer's instruction.

    Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.

    Techniques: Cell Culture, Transport Assay, Western Blot, Negative Control